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This invaluable reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact hematopoietic and lymphoid cells, and their maintenance as primary cultures. The text provides technical information on the signals and mediators required for the differentiation and growth of these cells, and is designed for laboratory investigators with limited practical experience in cell culture. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and the culturing of murine thymic explants. Each chapter has been written by experts who have practical experience of the techniques discussed to provide tips for avoiding common pitfalls, and sharing insight into the fundamental questions in cell biology and immunology addressed using each cell culture model.
- Sales Rank: #4202180 in Books
- Brand: Brand: Cambridge University Press
- Published on: 2000-08-28
- Original language: English
- Number of items: 1
- Dimensions: 8.98" h x .47" w x 5.98" l, .80 pounds
- Binding: Paperback
- 204 pages
- Used Book in Good Condition
Review
"The book would be of value to anyone who wishes to grow differentiated hematopoietic and lymphoid cells in culture...This is a useful, practical guide, is reasonably priced, and would be a valuable book to have on a laboratory bookshelf or in a library reference collection of techniques." Doody's Review Service ®
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